BSU18000

BSU_18000

2727

2730

BSU18000

BSU_18000

Citrate <=> isocitrate

Binding to iron responsive elements (IRE RNA) in the absence of the FeS cluster [Pubmed|23354745,10468622]

Citrate --> isocitrate (according to UniProt)

3-hydroxybutane-1,2,3-tricarboxylate --> 2-methyl-cis-aconitate + H₂O (according to UniProt)

Binding to iron responsive elements (IRE RNA) in the absence of the FeS cluster [Pubmed|23354745,10468622]

2-methylaconitate --> 2-methyl-isocitrate in the methylcitric acid cycle [pubmed|28956599]

FeS cluster

FeS cluster [pubmed|29292548]

[PDB|1L5J] (''E. coli'') [pubmed|11992126]

[PDB|2B3X] (the human enzyme, 53% identity) [pubmed|16407072]

GP1275 (Δ''citB''::''erm''), available in [SW|Jörg Stülke]'s lab

GP1441 (ΔcitB::spc), available in [SW|Jörg Stülke]'s lab

1A999 (''citB''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]

GP2338 (Δ''[[gene|citB]]''::''kan''), Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

GP2339 (Δ''[[gene|citB]]''::''lox72''), Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

GP1275 Δ''citB''::''erm'', available in [SW|Jörg Stülke]'s lab

GP1441 ΔcitB::spc, available in [SW|Jörg Stülke]'s lab

1A999 (''citB'')::''spec'', [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]

GP2338 Δ''[[gene|citB]]''::''kan'', Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

GP2339 Δ''[[gene|citB]]''::''lox72'', Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

BKE18000 (Δ[[gene|citB]]::erm trpC2)available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCAAAATCCCCCTT, downstream forward: _UP4_TGATGAATCAATAGGAAGAG

BKK18000 (Δ[[gene|citB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCAAAATCCCCCTT, downstream forward: _UP4_TGATGAATCAATAGGAAGAG

pGP700 (in [[protein|pAC5]]), available in [SW|Jörg Stülke]'s lab

pGP700 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

12732309, 2696478, 18261896, 18086213

12732309, 2696478, 18261896, 18086213, 32850966

18697947, 20097860, 2118511, 12850135, 2413006, 10656796, 10468622, 6143742, 16395550, 16923907, 9642180, 9393699, 12591885, 2105305, 20933603, 21099137, 21446632, 21821766, 22389480, 23139400, 23354745, 20817675, 15378759, 16923908, 21744456, 11992126

18697947, 20097860, 2118511, 12850135, 2413006, 10656796, 10468622, 6143742, 16395550, 16923907, 9642180, 9393699, 12591885, 2105305, 20933603, 21099137, 21446632, 21821766, 22389480, 23139400, 23354745, 20817675, 15378759, 16923908, 21744456, 11992126, 28956599, 16407072

aconitase/IPM isomerase family (with [[protein|LeuC]], according to UniProt)

[[this]]

pGP1810 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]